Organoid Research & 3D Cell Culture
What Comes Next After Successfully Creating Organoids?
— A Hands-on Seminar Exploring Reproducibility, Maturation, and Long-Term Culture Through the Culture Environment
As organoid research continues to expand, the focus is shifting beyond simply asking whether a 3D model can be created. Researchers are increasingly faced with questions such as whether organoids can be grown consistently, maintained over long periods, and used to generate comparable results across experiments.
From simply “creating” organoids to developing models that can be used reliably in research.
This seminar will explore reproducibility, maturation, and long-term maintenance in organoid culture through the approach of dynamic 3D cell culture. In addition to presentations, participants will have the opportunity to see, handle, and operate the system in a hands-on session.
Creating Organoids Is Only the Beginning
3D cell culture models such as organoids and spheroids are increasingly used across a wide range of research fields because they can reproduce cell–cell interactions and tissue structures that are difficult to capture in conventional 2D culture.
However, as these models move into practical research applications, additional challenges become apparent.
Reproducibility
Variations in organoid size or condition between experiments can make it difficult to compare and interpret results consistently.
Maturation
Even when a 3D structure has formed, it does not necessarily mean that the desired tissue functions or cellular states have fully matured.
Long-Term Culture
As 3D structures grow larger, oxygen and nutrient delivery to the interior, as well as waste removal, may influence the overall culture condition.
In other words, organoid research requires consideration not only of which cells and culture media are used, but also the environment in which the cells are grown.
Rethinking the Culture Environment
One approach to addressing these challenges is dynamic 3D cell culture, in which the culture medium is continuously moved while 3D structures are maintained in suspension.
Celvivo’s ClinoStar 2 is a dynamic culture system designed for 3D cell culture under low-shear conditions.
Dedicated bioreactors rotate gently to maintain cells and 3D structures in suspension. By creating a dynamic culture environment, the system is designed to reduce regions around the structures where nutrients and oxygen may become depleted.
Key Features of the ClinoStar 2 Dynamic 3D Culture Environment
- Dynamic 3D culture under low-shear conditions
- An approach designed to improve the nutrient and oxygen environment surrounding 3D structures
- Independent rotation control of six bioreactors
- Real-time monitoring using six cameras
- Temperature and CO₂ control, with optional hypoxic culture
- Support for remote operation and monitoring
How Can the Culture Environment Affect Research Results?
As 3D structures increase in size, it can become more difficult for nutrients and oxygen to reach their interior. Depending on the culture conditions, the internal environment of the structure may therefore differ from that at the surface.
Celvivo has published examples comparing brain organoids cultured using ClinoStar dynamic culture with those cultured using a conventional orbital shaker. These examples evaluate the use of ClinoStar for long-term brain organoid culture under low-shear conditions with dynamic mass transport, including assessments related to necrotic core formation.
It is important to note that no single system can solve every challenge in organoid culture.
Appropriate culture conditions vary depending on the cell type and research objective. The key is to consider what type of culture environment is most appropriate for each experimental model.
See, Touch, and Experience the System at This Seminar
Product catalogs and websites alone cannot fully convey how a system feels to operate, how the bioreactors are handled, or how it fits into a routine culture workflow.
At the 1st Yamato Scientific × VERITAS Hands-on Seminar, participants will learn about dynamic suspension culture for organoids through presentations and a practical hands-on session using the ClinoStar system.
Through live demonstrations and hands-on experience with the dedicated bioreactors, participants will be able to assess whether this approach may be suitable for their own research and how it differs from their current culture methods.
Seminar Program
Opening (13:30–13:35)
Session 1 (13:35–14:35)
“Expanding Applications of Dynamic Suspension Organoid Culture: From Toxicity Testing to Co-differentiation and Vascularized Models”
Speaker: Tomoko Birlund, Celvivo
Hands-on Session 1 (14:45–15:30)
“See and Experience a Dynamic Suspension Culture System for Organoids”
Session 2 (15:40–16:00)
“Introduction to Organoid-Related Products — ES/iPS Cell-Derived Neural Cells and Organoid-Related Products”
Speaker: Kanta Kozuka, VERITAS Corporation
Recommended for Researchers Who:
- Are already using organoids or spheroids in their research
- Are experiencing challenges with reproducibility or variability in 3D culture
- Are exploring long-term culture or enhanced maturation
- Are considering the use of 3D culture for toxicity testing, drug discovery, or disease modeling
- Would like to see and operate a dynamic suspension culture system firsthand
Event Details
| Seminar | 1st Yamato Scientific × VERITAS Hands-on Seminar Frontiers in Organoid Research — Latest Technologies and Applications Supporting Organoid Generation, Culture, and Maturation — |
|---|---|
| Date & Time | Friday, October 16, 2026, 13:30–16:00 |
| Venue | Life Innovation Center, 4F, Meeting Rooms A, B & C 3-25-22 Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa, Japan |
| Fee | Free |
| Capacity | 30 participants |
Could Rethinking the Culture Environment Help Address Your Research Challenges?
Reproducibility, maturation, and long-term culture may appear to be separate challenges in organoid research, but they are all closely related to the culture environment.
If you are experiencing variability under your current culture conditions or are seeking a more stable approach for maintaining 3D models over longer periods, it may be useful to reconsider not only the cells and media being used, but also the environment in which the cells are cultured.
This seminar offers an opportunity not only to learn about this approach through presentations, but also to see, handle, and experience the system firsthand.
Free Admission • Limited to 30 Participants
Discover a new option for organoid culture by seeing and experiencing the system firsthand.
Related Information
About This Article
This article has been prepared based on the official website and publicly available materials provided by Celvivo, the manufacturer of ClinoStar 2. Research examples and culture results described in this article may vary depending on the cell type, culture conditions, and experimental system used. For the latest product specifications and technical information, please refer to the Celvivo official website and current product documentation.
